EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.183 | - |
Rattus norvegicus |
EC Number | General Stability | Organism |
---|---|---|
2.7.1.60 | hexameric enzyme is completely stable with 0.1 mM UDP | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.183 | CMP-N-acetylneuraminic acid | - |
Rattus norvegicus | |
5.1.3.14 | CMP-N-acetylneuraminic acid | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.60 | 0.093 | - |
N-acetyl-D-mannosamine | pH 7.5, 37°C, hexamer | Rattus norvegicus | |
2.7.1.60 | 0.121 | - |
N-acetyl-D-mannosamine | pH 7.5, 37°C, dimer | Rattus norvegicus | |
2.7.1.60 | 1.18 | - |
ATP | pH 7.5, 37°C, hexamer | Rattus norvegicus | |
2.7.1.60 | 1.67 | - |
ATP | pH 7.5, 37°C, dimer | Rattus norvegicus | |
3.2.1.183 | 11 | - |
UDP-N-acetyl-alpha-D-glucosamine | pH 7.5, 37°C, hexamer | Rattus norvegicus | |
5.1.3.14 | 0.011 | - |
UDP-N-acetyl-D-glucosamine | bifunctional enzyme EC5.1.3.14/EC2.7.1.60 | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.60 | cytosol | - |
Rattus norvegicus | 5829 | - |
3.2.1.183 | cytosol | - |
Rattus norvegicus | 5829 | - |
5.1.3.14 | cytosol | - |
Rattus norvegicus | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.60 | 75000 | - |
gel filtration, SDS-PAGE | Rattus norvegicus |
2.7.1.60 | 150000 | - |
gel filtration, ion of the monomeric 75000 Da protein self associate as dimer | Rattus norvegicus |
2.7.1.60 | 450000 | - |
gel filtration, ion of the monomeric 75000 Da protein self associate as hexamer | Rattus norvegicus |
3.2.1.183 | 75000 | - |
monomer, SDS-PAGE, bifunctional enzymatic activity | Rattus norvegicus |
3.2.1.183 | 450000 | - |
gel filtration, dimer. Dimer does not show UDP-GlcNAc 2-epimerase activity. Incubation of the dimer with UDP-N-acetylglucosamine leads to reassembly of the fully active hexamer | Rattus norvegicus |
3.2.1.183 | 450000 | - |
gel filtration, hexamer, bifunctional enzyme complex | Rattus norvegicus |
5.1.3.14 | 450000 | - |
gel filtration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.60 | ATP + N-acetyl-D-mannosamine | Rattus norvegicus | - |
ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? | |
2.7.1.60 | ATP + N-acetyl-D-mannosamine | Rattus norvegicus Wistar | - |
ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.60 | Rattus norvegicus | - |
Wistar | - |
2.7.1.60 | Rattus norvegicus Wistar | - |
Wistar | - |
3.2.1.183 | Rattus norvegicus | - |
- |
- |
5.1.3.14 | Rattus norvegicus | - |
bifunctional enzyme EC 5.1.3.14/EC 2.7.1.60 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.60 | - |
Rattus norvegicus |
3.2.1.183 | using salmine sulfate precipitation and chromatography on phenyl-Sepharose, ATP-agarose, and Mono Q | Rattus norvegicus |
5.1.3.14 | bifunctional enzyme EC 5.1.3.14/EC 2.7.1.60 | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.60 | intestinal mucosa | - |
Rattus norvegicus | - |
2.7.1.60 | liver | - |
Rattus norvegicus | - |
2.7.1.60 | salivary gland | - |
Rattus norvegicus | - |
3.2.1.183 | liver | - |
Rattus norvegicus | - |
5.1.3.14 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.60 | 4.114 | - |
- |
Rattus norvegicus |
5.1.3.14 | 0.878 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.60 | -70°C, no loss of activity observed for several months | Rattus norvegicus |
5.1.3.14 | -70°C, stable for several months | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.60 | ATP + N-acetyl-D-mannosamine | - |
Rattus norvegicus | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? | |
2.7.1.60 | ATP + N-acetyl-D-mannosamine | - |
Rattus norvegicus Wistar | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? | |
2.7.1.60 | ATP + N-acetyl-D-mannosamine | - |
Rattus norvegicus | ADP + N-acetyl-D-mannosamine-6-phosphate | - |
? | |
2.7.1.60 | ATP + N-acetyl-D-mannosamine | - |
Rattus norvegicus Wistar | ADP + N-acetyl-D-mannosamine-6-phosphate | - |
? | |
2.7.1.60 | additional information | UDP-GlcNAc 2-epimerase and ManNAc kinase are parts of one bifunctional enzyme, catalyzes the first 2 steps in N-acetylneuraminic acid biosynthesis | Rattus norvegicus | ? | - |
? | |
2.7.1.60 | additional information | UDP-GlcNAc 2-epimerase and ManNAc kinase are parts of one bifunctional enzyme, catalyzes the first 2 steps in N-acetylneuraminic acid biosynthesis | Rattus norvegicus Wistar | ? | - |
? | |
3.2.1.183 | UDP-N-acetyl-alpha-D-glucosamine + H2O | - |
Rattus norvegicus | N-acetyl-alpha-D-mannosamine + UDP | - |
? | |
3.2.1.183 | UDP-N-acetyl-alpha-D-glucosamine + H2O | - |
Rattus norvegicus Wistar | N-acetyl-alpha-D-mannosamine + UDP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.60 | dimer | 2 * 75000, self associates as a dimer, gel filtration, SDS-PAGE, catalyzes only phosphorylation of N-acetylmannosamine, incubation with UDP-N-acetylglucosamine leads to reassembly of the fully active hexamer | Rattus norvegicus |
2.7.1.60 | hexamer | 6 * 75000, self associates as a hexamer, gel filtration, SDS-PAGE, catalyzes both enzyme activities, UDP-GlcNAc-epimerase/ManNAc kinase | Rattus norvegicus |
3.2.1.183 | dimer | 2 * 75000, gel filtration | Rattus norvegicus |
3.2.1.183 | hexamer | 6 * 75000, gel filtration | Rattus norvegicus |
5.1.3.14 | hexamer | bifunctional enzyme EC 5.1.3.14/EC 2.7.1.60, SDS-PAGE | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.60 | ManAc kinase | - |
Rattus norvegicus |
2.7.1.60 | UDP-GlcNAc-epimerase/ManNAc kinase | - |
Rattus norvegicus |
3.2.1.183 | UDP-GlcNAc 2-epimerase | bifunctional enzyme complex consisting of UDP-N-acetylglucosamine 2-epimerase and and N-acetylmannosamine kinase | Rattus norvegicus |
3.2.1.183 | UDP-N-acetylglucosamine 2-epimerase | - |
Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.183 | 37 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.183 | 7.5 | - |
assay at | Rattus norvegicus |